Difference between revisions of "Part:BBa K1760001:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Designed biobrick is a composite part that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD. In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzymes.
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This design contains;
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- a constitutive TetO promoter without TetR gene
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- an RBS designed by using Salis RBS calculator
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- a coding todE sequence codon optimized for E.Coli
  
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Constitutive TetO promoter without TetR gene. Gibson Assembled.
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This design is Gibson Assembled.
  
 
===Source===
 
===Source===

Revision as of 02:35, 19 September 2015


TodE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This design contains; - a constitutive TetO promoter without TetR gene - an RBS designed by using Salis RBS calculator - a coding todE sequence codon optimized for E.Coli

This design is Gibson Assembled.

Source

Pseudomonas putida F1 (Synthesized)

References