Difference between revisions of "Part:BBa K1728001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site (RBS)in the loop. There are three basic elements of toehold switches including 30bp trigger sequence(TTTCCCTTCATCTTTGAAGAAGAACCTATC), bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of Interleukin 1β (IL-1β) mRNA(IL1β partial sequence, BBa_K1728005). Once the IL-1β mRNA is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene. | Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site (RBS)in the loop. There are three basic elements of toehold switches including 30bp trigger sequence(TTTCCCTTCATCTTTGAAGAAGAACCTATC), bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of Interleukin 1β (IL-1β) mRNA(IL1β partial sequence, BBa_K1728005). Once the IL-1β mRNA is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene. | ||
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| + | == Toehold switch secondary structure prediction == | ||
| + | https://static.igem.org/mediawiki/2015/d/db/CGU_IL1B.jpg | ||
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Latest revision as of 02:32, 19 September 2015
IL1β toehold switch RNA sensor
IL1β toehold switch RNA sensor
Usage and Biology
Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site (RBS)in the loop. There are three basic elements of toehold switches including 30bp trigger sequence(TTTCCCTTCATCTTTGAAGAAGAACCTATC), bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of Interleukin 1β (IL-1β) mRNA(IL1β partial sequence, BBa_K1728005). Once the IL-1β mRNA is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene.
Toehold switch secondary structure prediction
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]