Difference between revisions of "Part:BBa K1680006"

Revision as of 02:32, 19 September 2015

Fluorescent protein dronpa

This part contains the protein coding sequence for a mutant (K145N) of the fluorescent protein Dronpa and is codon optimised for yeast. Dronpa is a photoswitchable protein. Fluorescence can be turned off by illumination with 488 nm light and switched back on by illumination with 405 nm. The K145N mutation leads to photoswitchable monomerisation/multimerisation behaviour (as described by Zhou et al. 2012). The part is supplied in RFC25. Fluorescence microscopy showed that the expression of this part works and photoswitching can be observed as expected.

Fluorescence, brightfield and overlay pictures of Saccharomyces cerevisiae wild type cells (top) and cells expressing dronpa from the pTUM104 plasmid (BBa_K801004) grown overnight in SC medium containing 2% galactose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 268
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 https://static.igem.org/mediawiki/2015/b/ba/Team_Tuebingen_microscpy-dronpa.png
-Fluorescence, brightfield and overlay pictures of ''Saccharomyces cerevisiae'' wild type cells (top) and cells expressing dronpa from the pTUM104 plasmid ([BBa_K801004]) grown overnight in SC medium containing 2% galactose.
+Fluorescence, brightfield and overlay pictures of ''Saccharomyces cerevisiae'' wild type cells (top) and cells expressing dronpa from the pTUM104 plasmid ([[BBa_K801004]]) grown overnight in SC medium containing 2% galactose.
 <!-- Add more about the biology of this part here