Difference between revisions of "Part:BBa K1820025"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1820025 short</partinfo>
 
<partinfo>BBa_K1820025 short</partinfo>
  
−
Add
+
A construct designed to kill ''Lactococcus lactis'' bacteria in the case of an infection by the P335 species lactococcal phage Φ31
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
The promoter in this construct is a divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31. It contains  a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes. Thus, when the phage infects the cell, it will activate the protein production that will kill the cell before the phage can successfully complete its life cycle.
  
 
<!-- -->
 
<!-- -->

Latest revision as of 02:28, 19 September 2015

p2001-native RBS-Fsp1-B0015

A construct designed to kill Lactococcus lactis bacteria in the case of an infection by the P335 species lactococcal phage Φ31

Usage and Biology

The promoter in this construct is a divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31. It contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes. Thus, when the phage infects the cell, it will activate the protein production that will kill the cell before the phage can successfully complete its life cycle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 541