Difference between revisions of "Part:BBa K1631003:Experience"

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We tried to confirm the quasi-lysis, but regardless of induction with 1mM IPTG, OD600 is decreased after about 4 hours. This maybe caused by the leak from lac promoter(BBa_R0011). It is considered that quasi-lysis is occuered at low expression level of Colicin Lysis Protein, and even if the expression level is bigger than that point, the effect to <i>E. coli</i> would not so change.
 
We tried to confirm the quasi-lysis, but regardless of induction with 1mM IPTG, OD600 is decreased after about 4 hours. This maybe caused by the leak from lac promoter(BBa_R0011). It is considered that quasi-lysis is occuered at low expression level of Colicin Lysis Protein, and even if the expression level is bigger than that point, the effect to <i>E. coli</i> would not so change.
  
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Reference
 
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<br>[1]Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubes, R., Postle, K., ... & Cavard, D. (2007). Colicin biology. Microbiology and Molecular Biology Reviews, 71(1), 158-229.
 
  
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===User Reviews===
 
===User Reviews===

Revision as of 02:25, 19 September 2015


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Applications of BBa_K1631003

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We tried to confirm the quasi-lysis, but regardless of induction with 1mM IPTG, OD600 is decreased after about 4 hours. This maybe caused by the leak from lac promoter(BBa_R0011). It is considered that quasi-lysis is occuered at low expression level of Colicin Lysis Protein, and even if the expression level is bigger than that point, the effect to E. coli would not so change.


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