Difference between revisions of "Part:BBa K1631003:Experience"

 
(→‎Applications of BBa_K1631003)
Line 5: Line 5:
  
 
===Applications of BBa_K1631003===
 
===Applications of BBa_K1631003===
 +
 +
 +
[[File:UT Tokyo ColL.png|600px|thumb|center|alt text]]
 +
 +
We tried to confirm the quasi-lysis, but regardless of induction with 1mM IPTG, OD600 is decreased after about 4 hours. This maybe caused by the leak from lac promoter(BBa_R0011). It is considered that quasi-lysis is occuered at low expression level of Colicin Lysis Protein, and even if the expression level is bigger than that point, the effect to <i>E. coli</i> would not so change.
 +
 +
Reference
 +
<br>[1]Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubes, R., Postle, K., ... & Cavard, D. (2007). Colicin biology. Microbiology and Molecular Biology Reviews, 71(1), 158-229.
 +
 +
<!-- -->
  
 
===User Reviews===
 
===User Reviews===

Revision as of 02:25, 19 September 2015


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1631003

alt text

We tried to confirm the quasi-lysis, but regardless of induction with 1mM IPTG, OD600 is decreased after about 4 hours. This maybe caused by the leak from lac promoter(BBa_R0011). It is considered that quasi-lysis is occuered at low expression level of Colicin Lysis Protein, and even if the expression level is bigger than that point, the effect to E. coli would not so change.

Reference
[1]Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubes, R., Postle, K., ... & Cavard, D. (2007). Colicin biology. Microbiology and Molecular Biology Reviews, 71(1), 158-229.


User Reviews

UNIQ9da7422ef39d1988-partinfo-00000000-QINU UNIQ9da7422ef39d1988-partinfo-00000001-QINU