Difference between revisions of "Part:BBa K1692033"

 
m
 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1692033 short</partinfo>
 
<partinfo>BBa_K1692033 short</partinfo>
  
This gene provides the intermediate for synthesizing gRNA targeting RFP (BBa_J04450). When used in conjuction with the CRISPR/Cas system, this gRNA will bind to and digest the RFP plasmid.
+
This gene provides the intermediate for synthesizing gRNA targeting RFP (BBa_J04450). After transcription, this gRNA in conjunction with the ''Streptococcus pyogenes'' Cas9 enzyme will bind to and cleave the RFP plasmid. To learn more about the function of this gRNA, refer to http://2015.igem.org/Team:Stanford-Brown/CRATER
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:20, 19 September 2015

RFP gDNA

This gene provides the intermediate for synthesizing gRNA targeting RFP (BBa_J04450). After transcription, this gRNA in conjunction with the Streptococcus pyogenes Cas9 enzyme will bind to and cleave the RFP plasmid. To learn more about the function of this gRNA, refer to http://2015.igem.org/Team:Stanford-Brown/CRATER

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]