Difference between revisions of "Part:BBa K1789007:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
  
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The length of DNA BMs and intervening sequences is designed based on the typical in vivo B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.
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The length of DNA BMs and intervening sequences is designed based on the typical ''in vivo'' B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.
  
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This scaffold is designed to test the effect of different length of intervening sequence. A 16-basis intervening sequence was added between BMs. The spatial orientation of enzymes is also considered.
+
This scaffold is designed to test the effect of different length of intervening sequence. A 16-bp intervening sequence was added between BMs. The spatial orientation of enzymes is also considered.
  
 
===Source===
 
===Source===

Latest revision as of 02:19, 19 September 2015


SCAF3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 8
    Illegal AgeI site found at 70
    Illegal AgeI site found at 132
    Illegal AgeI site found at 194
    Illegal AgeI site found at 256
    Illegal AgeI site found at 318
    Illegal AgeI site found at 380
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length of DNA BMs and intervening sequences is designed based on the typical in vivo B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.

This scaffold is designed to test the effect of different length of intervening sequence. A 16-bp intervening sequence was added between BMs. The spatial orientation of enzymes is also considered.

Source

This part is synthesized by company

References