Difference between revisions of "Part:BBa K1679019"
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<partinfo>BBa_K1679019 short</partinfo> | <partinfo>BBa_K1679019 short</partinfo> | ||
− | This is a Translational_Unit containing,a RBS (BBa_B0034),a GFP coding sequence (BBa_E0040) ,two terminator(BBa_B0010 and BBa_B0012),a promoter(BBa_J23119K),,a RNA thermometer ( | + | This is a Translational_Unit containing,a RBS (BBa_B0034),a GFP coding sequence (BBa_E0040) ,two terminator(BBa_B0010 and BBa_B0012),a promoter(BBa_J23119K),,a RNA thermometer (BBa_K115002) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli. |
This is Translational_Unit that a GFP reporter device except promoter in front of a RFP reprorter device. | This is Translational_Unit that a GFP reporter device except promoter in front of a RFP reprorter device. |
Revision as of 02:12, 19 September 2015
GFP+Promoter+RNA thermometer (FourU) +mRFP
This is a Translational_Unit containing,a RBS (BBa_B0034),a GFP coding sequence (BBa_E0040) ,two terminator(BBa_B0010 and BBa_B0012),a promoter(BBa_J23119K),,a RNA thermometer (BBa_K115002) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.
This is Translational_Unit that a GFP reporter device except promoter in front of a RFP reprorter device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 890
Illegal NheI site found at 913 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1539
Illegal AgeI site found at 1651 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 662