Difference between revisions of "Part:BBa K1820026"
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This part is a reporter sequence designed to produce superfolder green fluorescent protein when the host cell is infected by the P335 species lactococcal phage Φ31. | This part is a reporter sequence designed to produce superfolder green fluorescent protein when the host cell is infected by the P335 species lactococcal phage Φ31. | ||
Latest revision as of 02:06, 19 September 2015
p2001-native RBS-sfGFP-B0015
This part is a reporter sequence designed to produce superfolder green fluorescent protein when the host cell is infected by the P335 species lactococcal phage Φ31.
Usage and Biology
This construct is built from a promoter from the P335 species lactococcal phage Φ31, a native ribosome binding site, a sfGFP that is known to work well in Lactococcus lactis, and a double stop terminator. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 333