Difference between revisions of "Part:BBa K1602025"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1602025 short</partinfo>
 
<partinfo>BBa_K1602025 short</partinfo>
  
 +
<html>
 +
    <head>
 +
        <style>
 +
            table, td, th { border: 0px solid black; }
 +
        </style>
 +
    </head>
 +
    <body>
 +
   
 +
        <table>
 +
            <colgroup width=50% span="2">
 +
            <tr>
 +
                <td>
 +
                This part is encodes for the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield.
 +
                </td>
 +
               
 +
            </tr>
 +
            <tr>
 +
                <td>
 +
                    <!-- BLANK -->
 +
                </td>
 +
         
 +
            </tr>
 +
            </colgroup>
 +
        </table>
 +
    </body>
 +
</html>
  
  

Revision as of 02:04, 19 September 2015

HisTag-GFP_Si4-Tag fusion protein with RBS BBa_B0034

This part is encodes for the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 680