Difference between revisions of "Part:BBa K1820008"
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<partinfo>BBa_K1820008 short</partinfo> | <partinfo>BBa_K1820008 short</partinfo> | ||
− | Synthetic promoter created for use in ''Lactococcus lactis'' but also shows activity in ''E. coli'' from 2013 Uppsala team. Of the three promoters that we chose to use; cp8, cp11, and cp44; this one had the highest activity. It does not contain a ribosomal binding site. The paper listed below has more information. | + | Synthetic promoter created for use in ''Lactococcus lactis'' but also shows activity in ''E. coli'', part BBa_K1033220 from the 2013 Uppsala team. Of the three promoters that we chose to use; cp8, cp11, and cp44; this one had the highest activity. It does not contain a ribosomal binding site. The paper listed below has more information. |
Jensen, Peter Ruhdal and Karin Hammer. "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." Applied and Environmental Microbiology (1998): 82-87. | Jensen, Peter Ruhdal and Karin Hammer. "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." Applied and Environmental Microbiology (1998): 82-87. |
Revision as of 02:02, 19 September 2015
CP8 promoter
Synthetic promoter created for use in Lactococcus lactis but also shows activity in E. coli, part BBa_K1033220 from the 2013 Uppsala team. Of the three promoters that we chose to use; cp8, cp11, and cp44; this one had the highest activity. It does not contain a ribosomal binding site. The paper listed below has more information.
Jensen, Peter Ruhdal and Karin Hammer. "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." Applied and Environmental Microbiology (1998): 82-87. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]