Difference between revisions of "Part:BBa I13001"

Revision as of 01:58, 19 September 2015

RBS B0030 w/ YFP (-LVA) TT

B0030. E0030. B0015

Glasgow iGEM 2011

This part was found to express well when excited with fluorescence in the range of 515-565nm. See images below for expression testing:

Shenzhen_SFLS (IGEM 2015)

We expressed this part in E.coli using pbad promoter and J23116 promoter.

From left to right: J23116/pbad(0μM L-Arabinose)/0.5μM/1.0μM/2.0μM(best in the 5 concentrations we usd)/4.0μM

Sequence and Features

Assembly Compatibility:

@@ Line 17: / Line 17: @@
 We expressed this part in E.coli using pbad promoter and J23116 promoter.
-From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM
+From left to right: J23116/pbad(0μM L-Arabinose)/0.5μM/1.0μM/2.0μM(best in the 5 concentrations we usd)/4.0μM
 [[File:QQ图片20150210175650.jpg]] [[File:QQ图片20150210175655.jpg]]