Difference between revisions of "Part:BBa K1583061"
Line 10: Line 10:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K1583061 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1583061 SequenceAndFeatures</partinfo>
  

Revision as of 01:50, 19 September 2015

RBS

This RBS was used in our constructs Mfp5_CsgA (BBa_K1583104), CsgA_Mfp3 (BBa_K1583105) and BFP_SpyCatcher_His (BBa_K1583113).

CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces. This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.