Difference between revisions of "Part:BBa K1583061"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1583061 short</partinfo>
 
<partinfo>BBa_K1583061 short</partinfo>
 +
<html>
 +
<p>This RBS was used in our constructs Mfp5_CsgA (<a href="https://parts.igem.org/Part:BBa_K1583104"link+_blank">BBa_K1583104</a>), CsgA_Mfp3 (<a href="https://parts.igem.org/Part:BBa_K1583105"link+_blank">BBa_K1583105</a>) and BFP_SpyCatcher_His (<a href="https://parts.igem.org/Part:BBa_K1583113"link+_blank">BBa_K1583113</a>).</p>
  
−
This RBS was used in our constructs Mfp5+CsgA and CsgA+Mfp3.
+
<p>CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces.
−
CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces.
+
This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".</p>
−
This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
+
−
1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
+
  
−
 
+
</html>
−
<!-- Add more about the biology of this part here
+
−
===Usage and Biology===
+
  
 
<!-- -->
 
<!-- -->
Line 21: Line 18:
 
<partinfo>BBa_K1583061 parameters</partinfo>
 
<partinfo>BBa_K1583061 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<h3>References</h3>
 +
<p>C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.</p>

Revision as of 01:50, 19 September 2015

RBS

This RBS was used in our constructs Mfp5_CsgA (BBa_K1583104), CsgA_Mfp3 (BBa_K1583105) and BFP_SpyCatcher_His (BBa_K1583113).

CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces. This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.