Difference between revisions of "Part:BBa K1796012"
(→‎Parameter of Protein)
 
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==Parameter of Protein==
 
==Parameter of Protein==
−
<p> Number of amino acids: 136 </p>
+
<p> Number of amino acids: 128 </p>
 
<p> </p>
 
<p> </p>
−
<p> Molecular weight: 14908.1 </p>
+
<p> Molecular weight: 13989.1 </p>
 
<p> </p>
 
<p> </p>
−
<p> Theoretical pI: 6.48 </p>
+
<p> Theoretical pI: 6.47 </p>
 
<p> </p>
 
<p> </p>
−
<p> Amino acid composition:  </p>
+
<p> Amino acid composition:聽 </p>
 
<p> </p>
 
<p> </p>
−
<p> Ala (A)  15      11.0% </p>
+
<p> Ala (A)  15      11.7% </p>
 
<p> </p>
 
<p> </p>
−
<p> Arg (R)  9       6.6% </p>
+
<p> Arg (R)  7       5.5% </p>
 
<p> </p>
 
<p> </p>
−
<p> Asn (N)  4      2.9% </p>
+
<p> Asn (N)  4      3.1% </p>
 
<p> </p>
 
<p> </p>
−
<p> Asp (D)  3      2.2% </p>
+
<p> Asp (D)  3      2.3% </p>
 
<p> </p>
 
<p> </p>
−
<p> Cys (C)  2      1.5% </p>
+
<p> Cys (C)  1       0.8% </p>
 
<p> </p>
 
<p> </p>
−
<p> Gln (Q)  7      5.1% </p>
+
<p> Gln (Q)  7      5.5% </p>
 
<p> </p>
 
<p> </p>
−
<p> Glu (E)  13       9.6% </p>
+
<p> Glu (E)  11       8.6% </p>
 
<p> </p>
 
<p> </p>
−
<p> Gly (G)  12       8.8% </p>
+
<p> Gly (G)  10       7.8% </p>
 
<p> </p>
 
<p> </p>
−
<p> His (H)  4      2.9% </p>
+
<p> His (H)  4      3.1% </p>
 
<p> </p>
 
<p> </p>
−
<p> Ile (I)  11      8.1% </p>
+
<p> Ile (I)  11      8.6% </p>
 
<p> </p>
 
<p> </p>
−
<p> Leu (L)  10      7.4% </p>
+
<p> Leu (L)  10      7.8% </p>
 
<p> </p>
 
<p> </p>
−
<p> Lys (K)  6      4.4% </p>
+
<p> Lys (K)  6      4.7% </p>
 
<p> </p>
 
<p> </p>
−
<p> Met (M)  5       3.7% </p>
+
<p> Met (M)  4       3.1% </p>
 
<p> </p>
 
<p> </p>
−
<p> Phe (F)  7      5.1% </p>
+
<p> Phe (F)  7      5.5% </p>
 
<p> </p>
 
<p> </p>
−
<p> Pro (P)  4      2.9% </p>
+
<p> Pro (P)  4      3.1% </p>
 
<p> </p>
 
<p> </p>
−
<p> Ser (S)  8      5.9% </p>
+
<p> Ser (S)  8      6.2% </p>
 
<p> </p>
 
<p> </p>
−
<p> Thr (T)  6      4.4% </p>
+
<p> Thr (T)  6      4.7% </p>
 
<p> </p>
 
<p> </p>
−
<p> Trp (W)  1      0.7% </p>
+
<p> Trp (W)  1      0.8% </p>
 
<p> </p>
 
<p> </p>
 
<p> Tyr (Y)  0      0.0% </p>
 
<p> Tyr (Y)  0      0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Val (V)  9      6.6% </p>
+
<p> Val (V)  9      7.0% </p>
 
<p> </p>
 
<p> </p>
 
<p> Pyl (O)  0      0.0% </p>
 
<p> Pyl (O)  0      0.0% </p>
Line 78: Line 78:
 
<p> (X)  0          0.0% </p>
 
<p> (X)  0          0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Total number of negatively charged residues (Asp + Glu): 16 </p>
+
<p> Total number of negatively charged residues (Asp + Glu): 14 </p>
−
<p> Total number of positively charged residues (Arg + Lys): 15 </p>
+
<p> Total number of positively charged residues (Arg + Lys): 13 </p>
 
<p> </p>
 
<p> </p>
−
<p> Atomic composition:Carbon      C              655 </p>
+
<p> Atomic composition:Carbon      C              621 </p>
−
<p> Hydrogen    H         1057 </p>
+
<p> Hydrogen    H         999 </p>
−
<p> Nitrogen    N          189 </p>
+
<p> Nitrogen    N          175 </p>
−
<p> Oxygen      O          194 </p>
+
<p> Oxygen      O          182 </p>
−
<p> Sulfur      S            7 </p>
+
<p> Sulfur      S            5 </p>
 
<p> </p>
 
<p> </p>
−
<p> Formula: C655H1057N189O194S7Total number of atoms: 2102 </p>
+
<p> Formula: C621H999N175O182S5Total number of atoms: 1982 </p>
 
<p> </p>
 
<p> </p>
 
<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 
<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 
<p> </p>
 
<p> </p>
−
<p> Ext. coefficient    5625 </p>
+
<p> Ext. coefficient    5500 </p>
−
<p> Abs 0.1% (=1 g/l)  0.377, assuming all pairs of Cys residues form cystines </p>
+
<p> Abs 0.1% (=1 g/l)  0.393, assuming all pairs of Cys residues form cystines </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> Ext. coefficient    5500 </p>
 
<p> Ext. coefficient    5500 </p>
−
<p> Abs 0.1% (=1 g/l)  0.369, assuming all Cys residues are reduced </p>
+
<p> Abs 0.1% (=1 g/l)  0.393, assuming all Cys residues are reduced </p>
 
<p> </p>
 
<p> </p>
−
<p> Estimated half-life:The N-terminal of the sequence considered is C (Cys). </p>
+
<p> Estimated half-life:The N-terminal of the sequence considered is K (Lys). </p>
 
<p> </p>
 
<p> </p>
−
<p> The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro). </p>
+
<p> The estimated half-life is: 1.3 hours (mammalian reticulocytes, in vitro). </p>
−
<p>                             >20 hours (yeast, in vivo). </p>
+
<p>                             3 min (yeast, in vivo). </p>
−
<p>                             >10 hours (Escherichia coli, in vivo). </p>
+
<p>                             3 min (Escherichia coli, in vivo). </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
−
<p> Instability index:The instability index (II) is computed to be 46.59 </p>
+
<p> Instability index:The instability index (II) is computed to be 49.46 </p>
 
<p> This classifies the protein as unstable. </p>
 
<p> This classifies the protein as unstable. </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
−
<p> Aliphatic index: 90.44 </p>
+
<p> Aliphatic index: 96.09 </p>
 +
<p> </p>
 +
<p> Grand average of hydropathicity (GRAVY): 0.038 </p>
 +
<p> </p>
 +
<p> </p>
 
<p> </p>
 
<p> </p>
−
<p> Grand average of hydropathicity (GRAVY): -0.055 </p>
 

Latest revision as of 01:47, 19 September 2015

nifX promoted from Paenibacillus sp. WLY78

Function: encode carrier proteins involved in the delivery of FeMoco to the MoFe protein , participate in FeMoco assembly in a chaperone-like function.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 325
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 128

Molecular weight: 13989.1

Theoretical pI: 6.47

Amino acid composition:聽

Ala (A) 15 11.7%

Arg (R) 7 5.5%

Asn (N) 4 3.1%

Asp (D) 3 2.3%

Cys (C) 1 0.8%

Gln (Q) 7 5.5%

Glu (E) 11 8.6%

Gly (G) 10 7.8%

His (H) 4 3.1%

Ile (I) 11 8.6%

Leu (L) 10 7.8%

Lys (K) 6 4.7%

Met (M) 4 3.1%

Phe (F) 7 5.5%

Pro (P) 4 3.1%

Ser (S) 8 6.2%

Thr (T) 6 4.7%

Trp (W) 1 0.8%

Tyr (Y) 0 0.0%

Val (V) 9 7.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 14

Total number of positively charged residues (Arg + Lys): 13

Atomic composition:Carbon C 621

Hydrogen H 999

Nitrogen N 175

Oxygen O 182

Sulfur S 5

Formula: C621H999N175O182S5Total number of atoms: 1982

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 5500

Abs 0.1% (=1 g/l) 0.393, assuming all pairs of Cys residues form cystines

Ext. coefficient 5500

Abs 0.1% (=1 g/l) 0.393, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is K (Lys).

The estimated half-life is: 1.3 hours (mammalian reticulocytes, in vitro).

3 min (yeast, in vivo).

3 min (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 49.46

This classifies the protein as unstable.

Aliphatic index: 96.09

Grand average of hydropathicity (GRAVY): 0.038