Difference between revisions of "Part:BBa K1679005"

Revision as of 10:33, 16 September 2015 (view source) Registry (Talk | contribs)		Revision as of 01:46, 19 September 2015 (view source) QinQikai (Talk | contribs) Newer edit →
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	__NOTOC__		__NOTOC__
	<partinfo>BBa_K1679005 short</partinfo>		<partinfo>BBa_K1679005 short</partinfo>
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	This is a device that contain promoter BBa_J23117, RiboJ and BBa_I13504.		This is a device that contain promoter BBa_J23117, RiboJ and BBa_I13504.
−	<!-- Add more about the biology of this part here	+	===Plate Reader Result===
−	===Usage and Biology===	+
		+	<h3>Equipment</h3>
		+	<p>
		+	Varioskan Flash Multimode Reader<BR>Thermo Scientific<BR>Read Speed: 6.4 well per second
		+	</p>
		+	<h3>Software</h3>
		+	<p>
		+	Run Software Version: Skanlt Software 2.4.5 RE for Varioskan Flash<BR>Current Software Version: Skanlt Software 2.4.5 RE for Varioskan Flash
		+	</p>
		+	<h3>Protocol</h3>
		+	<p>
		+	Date: 2015 Aug 12<BR>1. Set our instrument to read OD600<BR>2. Setup a 96-well plate with our cultures<BR>3. Take the measurement and record it<BR>4. Calculate the dilution required for each sample (OD600=0.5)<BR>5. Dilute each sample<BR>6. Remeasure our sample on OD600<BR>7. Recalculate our dilution and remeasure until it's within 5% of 0.5.
		+	</p>
		+	<h3>Unit</h3>
		+	<p>
		+	Three technical replicates of M9 liquid media was measured, which were called background value.<BR>A series of concentration of sodium fluorescein was measured, which are 0, 10, 20, 40, 80, 160, 320, 640 ng/mL . A calibration curve was defined.
		+	</p>
		+	<div style="width: 50%;margin: 0 auto;">
		+	<center>https://static.igem.org/mediawiki/2015/f/f5/OUC-China-InterLab_2.jpg</center>
		+	</div>
		+	<h3>Dataset</h3>
		+	<p>
		+	All samples were cut the mean of background value, and then compared with the calibration curve to get the final dataset in units of fluorescein.
		+	</p>
		+	<div style="width: 50%;margin: 0 auto;">
		+	<center>https://static.igem.org/mediawiki/2015/b/bd/OUC-China-InterLab3.jpg</center>
		+	</div>
		+	<p></p>
		+	<div style="width: 80%;margin: 0 auto;">
		+	<center>https://static.igem.org/mediawiki/2015/c/c5/OUC-China-InterLab_4.jpg</center>
		+	</div>
		+	<p></p>
		+	<p>TR: Technical Replicate<BR>BR: Biological Replicate</p>
		+	<div style="width: 100%;margin: 0 auto;">
		+	<center>https://static.igem.org/mediawiki/2015/4/4e/OUC-China-InterLab_5.png</center>
		+	</div>
		+	<p></p>
	<!-- -->		<!-- -->

---

Revision as of 01:46, 19 September 2015

GFP Reporter

This is a device that contain promoter BBa_J23117, RiboJ and BBa_I13504.

Plate Reader Result

Equipment

Varioskan Flash Multimode Reader
Thermo Scientific
Read Speed: 6.4 well per second

Software

Run Software Version: Skanlt Software 2.4.5 RE for Varioskan Flash
Current Software Version: Skanlt Software 2.4.5 RE for Varioskan Flash

Protocol

Date: 2015 Aug 12
1. Set our instrument to read OD600
2. Setup a 96-well plate with our cultures
3. Take the measurement and record it
4. Calculate the dilution required for each sample (OD600=0.5)
5. Dilute each sample
6. Remeasure our sample on OD600
7. Recalculate our dilution and remeasure until it's within 5% of 0.5.

Unit

Three technical replicates of M9 liquid media was measured, which were called background value.
A series of concentration of sodium fluorescein was measured, which are 0, 10, 20, 40, 80, 160, 320, 640 ng/mL . A calibration curve was defined.

Dataset

All samples were cut the mean of background value, and then compared with the calibration curve to get the final dataset in units of fluorescein.

TR: Technical Replicate
BR: Biological Replicate

Sequence and Features