Difference between revisions of "Part:BBa K1602031"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K1602031 short</partinfo>
+
<partinfo>BBa_K1602030 short</partinfo>
−
 
+
<html>
−
 
+
    <head>
−
 
+
        <style>
−
 
+
            table, td, th { border: 0px solid black; }
−
<!-- Add more about the biology of this part here
+
        </style>
−
===Usage and Biology===
+
    </head>
−
 
+
    <body>
−
<!-- -->
+
   
−
<span class='h3bb'>Sequence and Features</span>
+
        <table>
−
<partinfo>BBa_K1602031 SequenceAndFeatures</partinfo>
+
            <colgroup width=50% span="2">
−
 
+
            <tr>
−
 
+
                <td>
−
<!-- Uncomment this to enable Functional Parameter display
+
                This part enables the production of the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(<a href:"https://parts.igem.org/Part:BBa_K1028000">BBa_K1028000</a>) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface.
−
===Functional Parameters===
+
                </td>
−
<partinfo>BBa_K1602031 parameters</partinfo>
+
               
−
<!-- -->
+
            </tr>
 +
            <tr>
 +
                <td>
 +
                    <!-- BLANK -->
 +
                </td>
 +
         
 +
            </tr>
 +
            </colgroup>
 +
        </table>
 +
    </body>
 +
</html>

Revision as of 01:35, 19 September 2015

HisTag-Scaffold_Si4-Tag fusion protein

This part enables the production of the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface.