Difference between revisions of "Part:BBa K1636000"
Line 19: Line 19:
  
 
The linker was ligated into the linearised plasmid pSB1C3 and previously cut with EcoRI and PstI, then a purification was performed (figure 1A). Several clones were selected to isolate plasmid by miniprep (figure 1). In order to analyse the integrity of plasmids an electrophoresis was run (figure 1B). Several plasmid isoforms were observed and the plasmids were suitable to be used as template in a PCR reaction to know the presence of the insert.
 
The linker was ligated into the linearised plasmid pSB1C3 and previously cut with EcoRI and PstI, then a purification was performed (figure 1A). Several clones were selected to isolate plasmid by miniprep (figure 1). In order to analyse the integrity of plasmids an electrophoresis was run (figure 1B). Several plasmid isoforms were observed and the plasmids were suitable to be used as template in a PCR reaction to know the presence of the insert.
 +
 +
https://static.igem.org/mediawiki/2015/a/a4/Aptamer_scheme_TecCEM.png
 +
'''Figure 3.''' A) Scheme of aptamer cloning in pSB1C3 plasmid and B) electrophoresis with agarose gel at 0.8% to analyse plasmid extraction from different clones (lanes 2 and 3 with 10 microliters of each sample + 4 microliters of loading buffer).

Revision as of 01:33, 19 September 2015

Lead-II-ions-specific aptamer

This aptameric sequence is capable of recognising and binding specifically to lead II ions. This sequence, which works as a single stranded nucleic acid molecule, is capable of forming a G-quadruplex by interacting with lead ions (Pb2+) while disrupting a partially complementary duplex.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


The linker was ligated into the linearised plasmid pSB1C3 and previously cut with EcoRI and PstI, then a purification was performed (figure 1A). Several clones were selected to isolate plasmid by miniprep (figure 1). In order to analyse the integrity of plasmids an electrophoresis was run (figure 1B). Several plasmid isoforms were observed and the plasmids were suitable to be used as template in a PCR reaction to know the presence of the insert.

Aptamer_scheme_TecCEM.png Figure 3. A) Scheme of aptamer cloning in pSB1C3 plasmid and B) electrophoresis with agarose gel at 0.8% to analyse plasmid extraction from different clones (lanes 2 and 3 with 10 microliters of each sample + 4 microliters of loading buffer).