Difference between revisions of "Part:BBa K1602030"

 
 
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<partinfo>BBa_K1602030 short</partinfo>
 
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                This part codes for the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(<a href:"https://parts.igem.org/Part:BBa_K1028000">BBa_K1028000</a>) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface.
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Latest revision as of 01:25, 19 September 2015

HisTag-Scaffold_Si4-Tag fusion protein

This part codes for the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 22
    Illegal BamHI site found at 877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 94
    Illegal AgeI site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]