Difference between revisions of "Part:BBa K1631011:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1631011=== | ===Applications of BBa_K1631011=== | ||
| + | We tried to confirm the quasi-lysis, but regardless of induction with 1mM IPTG, OD600 is decreased after about 4 hours. This maybe caused by the leak from lac promoter(BBa_R0011). It is considered that quasi-lysis is occuered at low expression level of Colicin Lysis Protein, and even if the expression level is bigger than that point, the effectiveness to E. coli is not so different. | ||
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| + | [[File:UT Tokyo ColL.png|600px|thumb|center|alt text]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 01:23, 19 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1631011
We tried to confirm the quasi-lysis, but regardless of induction with 1mM IPTG, OD600 is decreased after about 4 hours. This maybe caused by the leak from lac promoter(BBa_R0011). It is considered that quasi-lysis is occuered at low expression level of Colicin Lysis Protein, and even if the expression level is bigger than that point, the effectiveness to E. coli is not so different.
User Reviews
UNIQa381a733101f7e64-partinfo-00000000-QINU UNIQa381a733101f7e64-partinfo-00000001-QINU