Difference between revisions of "Part:BBa K1678004:Experience"

 
(Applications of BBa_K1678004)
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===Applications of BBa_K1678004===
 
===Applications of BBa_K1678004===
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The part was used in biobrick BBa_K1678006 as a promoter to express mCerulean. The promoter comes from <i>L.plantarum</i>, but also works in E.coli.
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We characterized the functioning of this promoter in <i>E.coli</i>. This promoter was put in front of mCerulean fluorescent protein, ligated into pSB1C3, and transformed into <i>E.coli</i>. The transformants were found to be fluorescing blue and were re-streaked with a non-fluorescent control on an LB agar plate.
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[[File:BBa_K1678004_plate.png|640px]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 01:22, 19 September 2015


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1678004

The part was used in biobrick BBa_K1678006 as a promoter to express mCerulean. The promoter comes from L.plantarum, but also works in E.coli.

We characterized the functioning of this promoter in E.coli. This promoter was put in front of mCerulean fluorescent protein, ligated into pSB1C3, and transformed into E.coli. The transformants were found to be fluorescing blue and were re-streaked with a non-fluorescent control on an LB agar plate.


BBa K1678004 plate.png

User Reviews

UNIQ797101fe5e0e3828-partinfo-00000000-QINU UNIQ797101fe5e0e3828-partinfo-00000001-QINU