Difference between revisions of "Part:BBa K1602028"

 
 
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<partinfo>BBa_K1602028 short</partinfo>
 
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                This part is capable of generating the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. The sequence coding for a His-tag is fused to the gene sequence, improving the purification yield. Also the production is codon optimized for growth in <i>E. coli</i> using part <a href=" https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
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Latest revision as of 01:21, 19 September 2015

Protein scaffold with N-terminal HisTag and RBS BBa_B0034

This part is capable of generating the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. The sequence coding for a His-tag is fused to the gene sequence, improving the purification yield. Also the production is codon optimized for growth in E. coli using part BBa_B0034.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 40
    Illegal BamHI site found at 895
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 112
    Illegal AgeI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]