Difference between revisions of "Part:BBa K1602027"

 
 
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<partinfo>BBa_K1602027 short</partinfo>
 
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                This part is capable of generating the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1602026 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1602026 parameters</partinfo>
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Latest revision as of 01:17, 19 September 2015

Protein scaffold with N-terminal HisTag

This part is capable of generating the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 22
    Illegal BamHI site found at 877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 94
    Illegal AgeI site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]