Difference between revisions of "Part:BBa K1728018:Design"

(→‎References)
(→‎Source)
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
DUSP1 toehold switch RNA sensor with T7 promoter (order from synthesis company) assembled with luciferase reporter (BBa_I712019) at the downstream.
+
DUSP1 toehold switch RNA sensor with T7 promoter (order from synthesis company) assembled with luciferase reporter (BBa_I712019) at the upstream.
  
 
===References===
 
===References===
 
Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin Toehold Switches: De-Novo-Designed Regulators of Gene Expression Cell.2014;159:925-939
 
Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin Toehold Switches: De-Novo-Designed Regulators of Gene Expression Cell.2014;159:925-939

Latest revision as of 01:17, 19 September 2015


DUSP1 toehold switch RNA sensor with T7 promoter & luciferase reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 47
    Illegal SapI.rc site found at 922


Design Notes

No.


Source

DUSP1 toehold switch RNA sensor with T7 promoter (order from synthesis company) assembled with luciferase reporter (BBa_I712019) at the upstream.

References

Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin Toehold Switches: De-Novo-Designed Regulators of Gene Expression Cell.2014;159:925-939