Difference between revisions of "Part:BBa K1640019"
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<partinfo>BBa_K1640019 short</partinfo>
 
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===Usage and Biology===
 
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ChlH is the catalytic subunit of Magnesium chelatase. This oligomeric enzyme initiates the first committed step of the chlorophyll-a biosynthesis pathway via insertion of an Mg2+ ion into protoporphyrin IX to generate Mg-protoporphyrin IX. Specifically, ChlH is the subunit known to bind porphyrin, and potentially also the Mg2+ ion. During this process, ChlH interacts with two AAA ATPase-like subunits of Mg-chelatase (ChlI and ChlD) to catalyse the ATP-dependent insertion of Mg2+ into protoporphyrin IX
 
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<p>The 4166 bp ChlH gene was engineered synthetically by Integrated DNA Technologies (IDT) in 3 gene blocks (Table 1). The original gene sequence was taken from <i>Chlamydomonas Reinhardtii</i> and subsequently codon optimized for expression in <i>Escherichia coli.</i> Integrity of the protein sequence was closely maintained throughout this optimisation process, but translation of the original clone and the synthesised sequences has revealed one mutation (‘E’ → ‘D’; ‘GAG’ → ‘GAT’). </p>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1640019 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1640019 parameters</partinfo>
 
<partinfo>BBa_K1640019 parameters</partinfo>
 
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===Overview===
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===Biology & Literature===
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===Protein information===
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===References===

Revision as of 01:03, 19 September 2015

Sequence and Features No part name specified with partinfo tag.


Overview

Biology & Literature

Protein information

References