Difference between revisions of "Part:BBa K1649003"

 
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<partinfo>BBa_K1649003 short</partinfo>
 
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GFP is used to report the function of blue light sensor. The LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL.
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GFP is used to report the function of blue light sensor. The LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL.The sensor is inactive under blue light and active in dark.
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===Measurement===
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<p>In order to test whether this part works,we cloned this part into pET28a using the NotI and XbaI sites. This plasmid contains a Kanamycin resistance gene and a pBR322 origin. BL21 strains are used to harbor blue light sensor plasmid.We measured OD600 and fluorescence of the strains at intervals. </p>
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[[file:NCTU_result_Pred_biobrick.png|thumb|600px|center|Figure 2. '''Notice that Pred includes a 37<sup>o</sup>C RBS that is only activated above or at 37<sup>o</sup>C.''' ]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:47, 19 September 2015

YF1 blue light sensor with GFP reporter

GFP is used to report the function of blue light sensor. The LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL.The sensor is inactive under blue light and active in dark.

Measurement

In order to test whether this part works,we cloned this part into pET28a using the NotI and XbaI sites. This plasmid contains a Kanamycin resistance gene and a pBR322 origin. BL21 strains are used to harbor blue light sensor plasmid.We measured OD600 and fluorescence of the strains at intervals.

Figure 2. Notice that Pred includes a 37oC RBS that is only activated above or at 37oC.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218
    Illegal BsaI.rc site found at 2904