Difference between revisions of "Part:BBa K1631012"

Revision as of 00:40, 19 September 2015

Plac -rbs- Barnase -rbs- GFP -d.term

Barnase and GFP is co-expressed by IPTG induction. This is composite parts of BBa_K1631000 which is improvement of BBa_I76211.

If you use this part, you can estimated the expression level of barnase and evaluate its toxic activity correspond to its expression level.

We succeeded to control growth rate of E coli.

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.

alt text

We also evaluated the growth rate by slope of next graph.

alt text

Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min) Sequence and Features

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1631012 short</partinfo>
-Barnase and GFP is co-expressed by IPTG induction.
+Barnase and GFP is co-expressed by IPTG induction. This is composite parts of BBa_K1631000 which is improvement of BBa_I76211.
 <br>
 <br>
@@ Line 9: / Line 9: @@
 <br>
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+We succeeded to control growth rate of <i>E coli</i>.
+<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
+[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
+</p>
+We also evaluated the growth rate by slope of next graph.
+[[File:Yamanaka 001.png|600px|thumb|center|alt text]]
+<br>Plac GFP-          0.0213(/min)
+<br>Plac GFP+          0.0205(/min)
+<br>Plac Barnase GFP-  0.0222(/min)
+<br>Plac Barnase GFP+  0.0117(/min)
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>