Difference between revisions of "Part:BBa K1631024"
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When this device works, Colicin-E3 will be released to media, and E. coli strains which does not have immunity protein will be killed. | When this device works, Colicin-E3 will be released to media, and E. coli strains which does not have immunity protein will be killed. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Colicins are a cytotoxins which are released to environment and kill other related strains. | ||
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| + | Release of colicin involves one protein; Colicin Lysis Protein. Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permiability and cause quasilysis.[1] After Colicin released, they diffuse through the medium and bind to the receptor on the target cell membrane. Then, they are imported to the cytoplasm or cytoplasmic membrane of target cell by Tol-system or Ton-system. (If you want to know the mechanism of the Colicin import, see [1]) | ||
| + | Colicin producing cells also express Colicin Immunity Protein in order to protect themselves from cytotoxity of colicin. | ||
| + | Colicins have variety cytotoxity such as DNase activity, RNase activity or Pore forming across inner membrane. | ||
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| + | Reference | ||
| + | [1]Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubes, R., Postle, K., ... & Cavard, D. (2007). Colicin biology. Microbiology and Molecular Biology Reviews, 71(1), 158-229. | ||
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Revision as of 00:37, 19 September 2015
Colicin-E3 cassette (Strong)
Colicin-E3, its immunity protein, and colicin lysis protein is constitutively expressed strong.(under BBa_?J23101) When this device works, Colicin-E3 will be released to media, and E. coli strains which does not have immunity protein will be killed.
Usage and Biology
Colicins are a cytotoxins which are released to environment and kill other related strains.
Release of colicin involves one protein; Colicin Lysis Protein. Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permiability and cause quasilysis.[1] After Colicin released, they diffuse through the medium and bind to the receptor on the target cell membrane. Then, they are imported to the cytoplasm or cytoplasmic membrane of target cell by Tol-system or Ton-system. (If you want to know the mechanism of the Colicin import, see [1]) Colicin producing cells also express Colicin Immunity Protein in order to protect themselves from cytotoxity of colicin. Colicins have variety cytotoxity such as DNase activity, RNase activity or Pore forming across inner membrane.
Reference [1]Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubes, R., Postle, K., ... & Cavard, D. (2007). Colicin biology. Microbiology and Molecular Biology Reviews, 71(1), 158-229.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1733
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 696
Illegal AgeI site found at 68
Illegal AgeI site found at 179
Illegal AgeI site found at 448
Illegal AgeI site found at 1714
Illegal AgeI site found at 1819 - 1000COMPATIBLE WITH RFC[1000]