Difference between revisions of "Part:BBa K1642010:Design"
(→‎Design Notes)
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
PcpcG2(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Control_System)
+
PcpcG2(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters)
  
 
HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)
 
HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)
  
−
PcpcG2-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Design)
+
PcpcG2-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Desalination_Process)
  
 
===Source===
 
===Source===
  
 
Overlap PCR from basic parts Up(BBa_K1642005), PcpcG2(BBa_K1642002), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).
 
Overlap PCR from basic parts Up(BBa_K1642005), PcpcG2(BBa_K1642002), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).

Latest revision as of 00:37, 19 September 2015

Expression of Halorhodopsin under the control of PcpcG2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 966
    Illegal NgoMIV site found at 1125
    Illegal NgoMIV site found at 1446
    Illegal AgeI site found at 1317
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PcpcG2(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters)

HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)

PcpcG2-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Desalination_Process)

Source

Overlap PCR from basic parts Up(BBa_K1642005), PcpcG2(BBa_K1642002), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).