Difference between revisions of "Part:BBa K1642009:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Pdark(http://2015.igem.org/Team:SJTU-BioX-Shanghai/ | + | Pdark(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters) |
Pdark-GFP | Pdark-GFP | ||
Latest revision as of 00:33, 19 September 2015
Expression of GFP under the control of Pdark
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 611
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2995
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 481
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1294
Design Notes
Pdark(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters)
Pdark-GFP
Source
ClonExpress technique of Vazyme Biotech Co.,Ltd from composite Pdark-HR(BBa_K1642011) and GFP gene sequence.