Difference between revisions of "Part:BBa K1675004"
 
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<partinfo>BBa_K1675004 short</partinfo>
 
<partinfo>BBa_K1675004 short</partinfo>
  
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The strong constitutive promoter J23119, the ribosome binding site B0034 and the functional gene ''NhaA''([BBa_K1675000]) were used in this new standard part. This standard part is available for the bacteria to survive in alkaline environment.
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The strong constitutive promoter J23119, the ribosome binding site B0034 and the functional gene ''NhaA''(BBa_K1675000) were used in this new standard part. This standard part is available for the bacteria to survive in alkaline environment.
  
 
We designed suitable primers and linked JBA (J23119+B0034+''NhaA'') with pSB1C3. The agarose gel electrophoresis analysis below (Fig.1) shows the positive clones of JBA.
 
We designed suitable primers and linked JBA (J23119+B0034+''NhaA'') with pSB1C3. The agarose gel electrophoresis analysis below (Fig.1) shows the positive clones of JBA.

Latest revision as of 00:31, 19 September 2015

J23119+B0034+NhaA(BBa_K1675000)

The strong constitutive promoter J23119, the ribosome binding site B0034 and the functional gene NhaA(BBa_K1675000) were used in this new standard part. This standard part is available for the bacteria to survive in alkaline environment.

We designed suitable primers and linked JBA (J23119+B0034+NhaA) with pSB1C3. The agarose gel electrophoresis analysis below (Fig.1) shows the positive clones of JBA.

Fig.5-a.png

Fig.1 The positive clones of JBA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BamHI site found at 1058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 384
  • 1000
    COMPATIBLE WITH RFC[1000]