Difference between revisions of "Part:BBa K1813024"
 
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<partinfo>BBa_K1813024 short</partinfo>
 
<partinfo>BBa_K1813024 short</partinfo>
  
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A construct used to achieve the bacterial expression of HUMCYPDB1 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</b></a></html>) under regulative authority of the LacI repressor.
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A construct used to achieve the bacterial expression of CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</b></a></html>) under regulative authority of the LacI repressor.
  
  
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<h2> Background </h2>
 
<h2> Background </h2>
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Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of HUMCYPDB1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.
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Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP2D6 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.

Latest revision as of 00:31, 19 September 2015

CYP2D6 Expression Cassette with LacI Reversed

A construct used to achieve the bacterial expression of CYP2D6 (BBa_K1813005) under regulative authority of the LacI repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 1737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2327
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP2D6 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.