Difference between revisions of "Part:BBa K1675004"

 
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<partinfo>BBa_K1675004 short</partinfo>
 
<partinfo>BBa_K1675004 short</partinfo>
  
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The constitutive promoter J23119, the ribosome binding site B0034 and the functional gene NhaA were used in this new standard part. This part is available for the bacteria to survive in alkaline environment.
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The strong constitutive promoter J23119, the ribosome binding site B0034 and the functional gene ''NhaA''([BBa_K1675000]) were used in this new standard part. This standard part is available for the bacteria to survive in alkaline environment.
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We designed suitable primers and linked JBA (J23119+B0034+''NhaA'') with pSB1C3. The agarose gel electrophoresis analysis below (Fig.1) shows the positive clones of JBA.
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[[File:Fig.5-a.png]]
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Fig.1 The positive clones of JBA
  
 
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Revision as of 00:30, 19 September 2015

J23119+B0034+NhaA(BBa_K1675000)

The strong constitutive promoter J23119, the ribosome binding site B0034 and the functional gene NhaA([BBa_K1675000]) were used in this new standard part. This standard part is available for the bacteria to survive in alkaline environment.

We designed suitable primers and linked JBA (J23119+B0034+NhaA) with pSB1C3. The agarose gel electrophoresis analysis below (Fig.1) shows the positive clones of JBA.

Fig.5-a.png

Fig.1 The positive clones of JBA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BamHI site found at 1058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 384
  • 1000
    COMPATIBLE WITH RFC[1000]