Difference between revisions of "Part:BBa K1583016"

 
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<partinfo>BBa_K1583016 short</partinfo>
 
<partinfo>BBa_K1583016 short</partinfo>
  
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CsgA is a protein monomer which can aggregate to foCsgB functions as the anchor on the cell wall for curli nanowires. It triggers the aggregation of CsgA and leads to the formation of amyloid nanowires. CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA.  
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CsgA is a protein monomer which can aggregate to form amyloid nanowires after nucleation on CsgB, a membrane protein in natural biofilms of <i>E.coli</i> (Barnhart & Chapman 2010).</p>
  
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<p><i>csgB</i> is used in the biobricks:
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===Usage and Biology===
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<ul><li><a href="https://parts.igem.org/Part:BBa_K1583203"link+_blank">BBa_K1583203</a></li>
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<li><a href="https://parts.igem.org/Part:BBa_K1583204"link+_blank">BBa_K1583204</a></li>
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<li><a href="https://parts.igem.org/Part:BBa_K1583205"link+_blank">BBa_K1583205</a></li></ul></p>
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<p>These three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).</p>
  
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K1583016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1583016 SequenceAndFeatures</partinfo>
  
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<h3>References</h3>
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<p>Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.</p>
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===Functional Parameters===
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<partinfo>BBa_K1583016 parameters</partinfo>
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Revision as of 00:22, 19 September 2015

CsgB

CsgA is a protein monomer which can aggregate to form amyloid nanowires after nucleation on CsgB, a membrane protein in natural biofilms of E.coli (Barnhart & Chapman 2010).</p>

csgB is used in the biobricks:

These three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.