Difference between revisions of "Part:BBa K1583001"
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<i>csgC</i> is used in the biobricks:
 
<i>csgC</i> is used in the biobricks:
 
<ul><li><a href="https://parts.igem.org/Part:BBa_K1583200"link+_blank">BBa_K1583200</a></li>
 
<ul><li><a href="https://parts.igem.org/Part:BBa_K1583200"link+_blank">BBa_K1583200</a></li>
−
<li><a href="https://parts.igem.org/Part:BBa_K1583201"link+_blank">>BBa_K1583201</a></li>
+
<li><a href="https://parts.igem.org/Part:BBa_K1583201"link+_blank">BBa_K1583201</a></li>
−
<li><a href="https://parts.igem.org/Part:BBa_K1583202"link+_blank">>BBa_K1583202</a></li>
+
<li><a href="https://parts.igem.org/Part:BBa_K1583202"link+_blank">BBa_K1583202</a></li>
−
<li><a href="https://parts.igem.org/Part:BBa_K1583203"link+_blank">>BBa_K1583203</a></li>
+
<li><a href="https://parts.igem.org/Part:BBa_K1583203"link+_blank">BBa_K1583203</a></li>
−
<li><a href="https://parts.igem.org/Part:BBa_K1583204"link+_blank">>BBa_K1583204</a></li>
+
<li><a href="https://parts.igem.org/Part:BBa_K1583204"link+_blank">BBa_K1583204</a></li>
−
<li><a href="https://parts.igem.org/Part:BBa_K1583205"link+_blank">>BBa_K1583205</a></li></ul>
+
<li><a href="https://parts.igem.org/Part:BBa_K1583205"link+_blank">BBa_K1583205</a></li></ul>
 
   
 
   
 
The first three parts contain CsgC with a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).
 
The first three parts contain CsgC with a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).

Revision as of 00:17, 19 September 2015

CsgC

CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli (Barnhart & Chapman 2010). csgC is used in the biobricks:

The first three parts contain CsgC with a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100). The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.