Difference between revisions of "Part:BBa K1583001"
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<partinfo>BBa_K1583001 short</partinfo>
 
<partinfo>BBa_K1583001 short</partinfo>
  
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CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of <i>E.coli</i> (Barnhart & Chapman 2010).
 
CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of <i>E.coli</i> (Barnhart & Chapman 2010).
  
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The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).
 
The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 00:14, 19 September 2015

CsgC

CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli (Barnhart & Chapman 2010). It is used in the biobricks:

The first three parts contain CsgC with a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100). The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.