Difference between revisions of "Part:BBa K1583001"
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<partinfo>BBa_K1583001 short</partinfo>
 
<partinfo>BBa_K1583001 short</partinfo>
  
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CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of <i>E.coli</i> (Barnhart & Chapman 2010).  
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CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of <i>E.coli</i> (Barnhart & Chapman 2010).
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It is used in the biobricks:
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<ul><li><a href="https://parts.igem.org/Part:BBa_K1583200">BBa_K1583200</a>
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<li><a href="https://parts.igem.org/Part:BBa_K1583201">BBa_K1583201</a><li/>
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<li><a href="https://parts.igem.org/Part:BBa_K1583202">BBa_K1583202</a><li/>
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<li><a href="https://parts.igem.org/Part:BBa_K1583203">BBa_K1583203</a><li/>
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<li><a href="https://parts.igem.org/Part:BBa_K1583204">BBa_K1583204</a><li/>
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<li><a href="https://parts.igem.org/Part:BBa_K1583205">BBa_K1583205</a><li/></ul>
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The first three parts contain CsgC with a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).
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The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).
  
 
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Revision as of 00:13, 19 September 2015

CsgC

CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli (Barnhart & Chapman 2010).

It is used in the biobricks:

The first three parts contain CsgC with a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).


The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.