Difference between revisions of "Part:BBa K632003:Experience"

(→‎User Reviews)
(→‎User Reviews)
Line 17: Line 17:
 
We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light.
 
We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light.
 
We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator.
 
We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator.
 +
https://static.igem.org/mediawiki/2015/f/f2/Team_HZAU-China_Fig_W4-1.png
 +
https://static.igem.org/mediawiki/2015/a/a7/Team_HZAU-China_Fig_W4-2.png
 
Fig 1: The genetic circuit of test system.
 
Fig 1: The genetic circuit of test system.
 
<br>
 
<br>

Revision as of 23:55, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K632003

User Reviews

UNIQ21825ce0a36264ec-partinfo-00000000-QINU

No review score entered. HZAU-China iGEM 2015


We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light. We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator. Team_HZAU-China_Fig_W4-1.png Team_HZAU-China_Fig_W4-2.png Fig 1: The genetic circuit of test system.
Two sample owning the same genetic circuit were set in our test experiment, the one in dark, the other in red light, and each sample had three biologic repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture shown. Fig 2:A red LED powered by battery is set at the bottom of a cup to provide red light Fig 3:Two light conditions in our test device.
After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes. (In the test, we used GFP as the target gene). Fig 4. The effect of red light on the expression of genes
More information, click here: http://2015.igem.org/Team:HZAU-China

;

UNIQ21825ce0a36264ec-partinfo-00000002-QINU