Difference between revisions of "Part:BBa K1758100"

Revision as of 23:39, 18 September 2015

Translation enhancing 5-UTR containing g10-L RBS

This sequence contains a 5' untranslated region (5'-UTR) and a strong ribosomal binding site from bacteriophage T7, named g10-L. This sequence greatly enhances translation of a following gene. The enhancing effect relies on the regulation of mRNA binding to and release of the ribosome S30 subunit (for details see [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).

g10-L is a strong RBS in E. coli but also well suited for foreign gene expression ([http://www.nature.com/nbt/journal/v9/n5/abs/nbt0591-477.html Rangwala et al. 1991]).

Usage and Biology

This part includes a RBS and a translation enhancing sequence. It improves the expression of a following gene when cloned in front of it compared to other RBS, for example B0034. The sequence of this part is derived from Olins et al. 1989 and ribosome binding analysis from Takahashi et al. 2013.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ''g10''-L is a strong RBS in ''E. coli'' but also well suited for foreign gene expression ([http://www.nature.com/nbt/journal/v9/n5/abs/nbt0591-477.html Rangwala et al. 1991]).
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 ===Usage and Biology===
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 <p> This part includes a RBS and a translation enhancing sequence. It improves the expression of a following gene when cloned in front of it compared to other RBS, for example B0034.