Difference between revisions of "Part:BBa K1758100"

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===Usage and Biology===
 
===Usage and Biology===
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<p> This part includes a RBS and a translation enhancing sequence. It improves the expression of a following gene when cloned in front of it compared to other RBS, for example B0034.
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The sequence of this part is derived from <a href="http://www.ncbi.nlm.nih.gov/pubmed/2676996">Olins et al. 1989</a> and ribosome binding analysis from <a href="http://www.ncbi.nlm.nih.gov/pubmed/23927491">Takahashi et al. 2013</a>.
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Revision as of 23:38, 18 September 2015

Translation enhancing 5-UTR containing g10-L RBS

This sequence contains a 5' untranslated region (5'-UTR) and a strong ribosomal binding site from bacteriophage T7, named g10-L. This sequence greatly enhances translation of a following gene. The enhancing effect relies on the regulation of mRNA binding to and release of the ribosome S30 subunit (for details see [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).

g10-L is a strong RBS in E. coli but also well suited for foreign gene expression ([http://www.nature.com/nbt/journal/v9/n5/abs/nbt0591-477.html Rangwala et al. 1991]).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]