Difference between revisions of "Part:BBa K1632002"
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<span style="margin-left: 10px;">The <i>fim</i> switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the <i>fim</i> switch by adding the Fim recombinase.<br> | <span style="margin-left: 10px;">The <i>fim</i> switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the <i>fim</i> switch by adding the Fim recombinase.<br> | ||
| − | <span style="margin-left: 10px;">We designed this ''fim'' switch which has a J23119 promoter. Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. ''fim'' switch (Tokyo_Tech/J23119) design). So the promoter can easily be interchanged. Except for insertion of restriction enzyme sites, basically, the design of <i>fim</i> switch(Tokyo_Tech) is similar with <i>fim</i> switch(wild-type). | + | <span style="margin-left: 10px;">We designed this ''fim'' switch which has a J23119 promoter(<partinfo>BBa_J23119</BBa_J23119>). Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. ''fim'' switch (Tokyo_Tech/J23119) design). So the promoter can easily be interchanged. Except for insertion of restriction enzyme sites, basically, the design of <i>fim</i> switch(Tokyo_Tech) is similar with <i>fim</i> switch(wild-type). |
[[Image:Tokyo Tech fim switch Tokyo Tech design.png|thumb|center|600px|Fig. 1. ''fim'' switch(Tokyo_Tech/J23119) design (Up:on state Down:off state)]]<br> | [[Image:Tokyo Tech fim switch Tokyo Tech design.png|thumb|center|600px|Fig. 1. ''fim'' switch(Tokyo_Tech/J23119) design (Up:on state Down:off state)]]<br> | ||
Revision as of 23:28, 18 September 2015
fim switch[default ON](Tokyo_Tech/J23119)_rbs_gfp
The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
We designed this fim switch which has a J23119 promoter(No part name specified with partinfo tag.