Difference between revisions of "Part:BBa K1807001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was sub-cloned from the pBC9 plasmid, first published by (). | + | This part was sub-cloned from the pBC9 plasmid, first published by (Akiyama et al., 1993). |
The primer pair used to PCR clone the part: | The primer pair used to PCR clone the part: | ||
Revision as of 23:27, 18 September 2015
Escherichia coli Exopolyphosphatase (PPX)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 872
Design Notes
This part was sub-cloned from the pBC9 plasmid, first published by (Akiyama et al., 1993).
The primer pair used to PCR clone the part:
Forward: AGCACATACGAGAAAGAGGAGAAATACcccATGCCAATACACGATAAATCCCCT
Reverse: GAGCCTTTCGTTTTATTTGATGCCTGGcccTTAAGCGGCGATTTCTGGTGTACT
Source
Escherichia coli BW25113