Difference between revisions of "Part:BBa K1807001"

Revision as of 23:22, 18 September 2015

Escherichia coli Exopolyphosphatase (PPX)

This part codes for the exopolyphosphatase (PPX) enzyme of Escherichia coli. PPX is able to release phosphate residues from the ends of a polyphosphate chain.

Usage and Biology

Exopolyphosphatase is an enzyme encoded by the PPX gene (Akiyama et al., 1993). Exopolyphosphatase cleaves phosphate residues from the termini of polyphosphate chains. Preferred substrate seems to be polyphosphate with approximately 500 residues long chain. ATP and short polyphosphate chains (around 15 residues) are either not a substrate at all or compete poorly with long polyphosphate.

Akiyama, M., E. Crooke, and A. Kornberg. "An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon." Journal of Biological Chemistry 268.1 (1993): 633-639.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
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 ===Usage and Biology===
-Exopolyphosphatase is an enzyme encoded by the PPX gene (Akiyama et al., 1993). Exopolyphosphatase cleaves phosphate residues from the termini of polyphosphate chains.
+Exopolyphosphatase is an enzyme encoded by the PPX gene (Akiyama et al., 1993). Exopolyphosphatase cleaves phosphate residues from the termini of polyphosphate chains. Preferred substrate seems to be polyphosphate with approximately 500 residues long chain. ATP and short polyphosphate chains (around 15 residues) are either not a substrate at all or compete poorly with long polyphosphate.
 Akiyama, M., E. Crooke, and A. Kornberg. "An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon." Journal of Biological Chemistry 268.1 (1993): 633-639.