Difference between revisions of "Part:BBa K1819000"
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| − | <p>This part was characterized using two constitutive promoters from Anderson library (BBa_J23106 and BBa_J23117). See BBa_K1819006 and BBa_K1819007 pages for more information.</p> | + | |
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| + | <p>We added this part as an improvement to BBa_E0040, a green fluorescente protein, for protein fusion (see <a href="https://parts.igem.org/Part:BBa_E0040:Experience>"Experience page</a>) . | ||
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| + | This part was characterized using two constitutive promoters from Anderson library (BBa_J23106 and BBa_J23117). See BBa_K1819006 and BBa_K1819007 pages for more information.</p> | ||
Revision as of 23:04, 18 September 2015
Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site.
It allows to characterize protein expression and secretion and also may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
We also added GGGS amino acid twice as a spacer to avoid interaction between two consecutive proteins, allowing proper folding. This strategy was already applied to BBa_K1489002 part.
Figure 1 - Schematic representation of BBa_K1819000 insert
Characterization
Figure 2 - Gel restriction analysis
We added this part as an improvement to BBa_E0040, a green fluorescente protein, for protein fusion (see ) .
This part was characterized using two constitutive promoters from Anderson library (BBa_J23106 and BBa_J23117). See BBa_K1819006 and BBa_K1819007 pages for more information.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 674