Difference between revisions of "Part:BBa K1758351:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1758351 short</partinfo>
 
<partinfo>BBa_K1758351 short</partinfo>
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===Design Notes===
 
===Design Notes===
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...
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We used the codon optimized, synthesized Sequence of RFP under the control of rcnA from iGEM Team BIT 2013(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K549001" target="_blank"> BBa_K549001 </a>). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of rcnA with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of rcnA  and the pSB1C3:
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank">cm_fwd</a></p>
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<p> CGGCATCAGCACCTTGTC </p>
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> pSB1C3_RFP_rev</a></p>
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<p>CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA</p>
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank">cm_rev</a></p>
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<p> TATACGCAAGGCGACAAG </p>
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_rcnA_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_rcnA_fwd" target="_blank"> pSB1C3_rcnA_fwd</a>
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</p> <p>TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT</p>
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===Source===
 
===Source===
  
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design based on K549001
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design based on BBa_K549001(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K549001" target="_blank"> BBa_K59001</a>)
  
 
===References===
 
===References===

Latest revision as of 22:59, 18 September 2015

Nickel responsive promoter with RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the codon optimized, synthesized Sequence of RFP under the control of rcnA from iGEM Team BIT 2013( BBa_K549001 ). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of rcnA with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of rcnA and the pSB1C3:

cm_fwd

CGGCATCAGCACCTTGTC

pSB1C3_RFP_rev

CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA

cm_rev

TATACGCAAGGCGACAAG

pSB1C3_rcnA_fwd

TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT


Source

design based on BBa_K549001(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K549001" target="_blank"> BBa_K59001</a>)

References