Difference between revisions of "Part:BBa K1585201"
(→‎Usage and Biology)
Line 21: Line 21:
 
[[File:Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png]]
 
[[File:Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png]]
  
−
Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
+
Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
  
  

Revision as of 22:59, 18 September 2015

HPS

3-hexulose-6-phosphate synthase from Bacillus methanolicus MGA3, codon optimized for E.coli


Catalysed reaction by Hps
Formaldehyde + Ribulose-5-phosphat → D-arabino-hex-3-ulose 6-phosphate.



Usage and Biology

Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]