Difference between revisions of "Part:BBa K1813029"
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<h2>Background of <i>nicF</i>:</h2>
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<h2>Background of <i>nicX</i>:</h2>
 
<h4>Part Description:</h4>
 
<h4>Part Description:</h4>
 
<p>Codon optimized coding sequence of <i>nicX</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813030">BBa_K1813030</a> </html> and  
 
<p>Codon optimized coding sequence of <i>nicX</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813030">BBa_K1813030</a> </html> and  

Revision as of 22:31, 18 September 2015

nicX

nicX

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 332
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal AgeI site found at 709
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]



Background of nicX:

Part Description:

Codon optimized coding sequence of nicX, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813030 and BBa_K1813031 .

Part Origin:

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function:

The enzyme coded for by nicX is a 2,5 DHP dioxygenase that converts 2,5 Dihydroxypyridine (2,5 DHP) to N-Formylmaleamic Acid (NFM).

References:

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105