Difference between revisions of "Part:BBa K1627003"
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===Usage and Biology===
 
===Usage and Biology===
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Revision as of 22:28, 18 September 2015

Device to demethylate theophylline

This part contains genes that allow for the demethylation of theophylline. The part contains a synthetic operon consisting of ndmA, ndmB, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.

This part can be used in a ∆guaB strain of E. coli to measure the amount of theophylline in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of theophylline, a growth curve can be made that correlates culture growth with concentration of theophylline. Then if the strain is grown in a minimal media with an unknown concentration of theophylline, the growth can be compared to the growth curve to calculate the initial concentration of theophylline in the media.

Usage and Biology

2015 Austin UTexas PDCAFAB growth curve.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1733
    Illegal NheI site found at 1928
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2296
    Illegal BglII site found at 2767
    Illegal XhoI site found at 3908
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4606
    Illegal AgeI site found at 2435
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 983