Difference between revisions of "Part:BBa K1807002"

Revision as of 22:27, 18 September 2015

Escherichia coli Polyphosphate Kinase Enzyme

This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli.

Usage and Biology

PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.

Akiyama, Masahiro, E. Crooke, and Arthur Kornberg. "The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein." Journal of Biological Chemistry 267.31 (1992): 22556-22561.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 396
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1750
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1807002 short</partinfo>
-This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from ''Escherichia coli''. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the &#947;-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.
+This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from ''Escherichia coli''.
 ===Usage and Biology===
+PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the &#947;-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.
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